Route to Eastlake Virology (EVIR rack 81). Immunogenicity and protective efficacy of SARS-CoV-2 mRNA vaccine encoding secreted non-stabilized spike in female mice. Nature Communications (Nat Commun) The promising preclinical study results presented here demonstrate that ChulaCov19 is highly immunogenic with protective efficacy. Zhang, N. N. et al. After 1h incubation at 37C, plates were washed vigorously with washing buffer (PBS+0.5% Tween 20, PBST). The score (0-5) was assigned according to the percent distribution of fluorescent-positive cells. E.P., C.K., D.W., and K.R. Different studies have used different methods to measure antibody levels, making it difficult to compare results and establish a universal cutoff value conferring protection in immunocompromised patients. Recombinant S protein with abolished S1/S2 cleavage site was used as positive control in HEK293T-hACE-2 binding assay (right panel of 2b) and western blot (right lane of each panel in 2c). Nature 589, 603607 (2021). World Health Organization. In these preclinical studies in mice, we have demonstrated that ChulaCov19, a secreted, prefusion non-stabilized ectodomain spike mRNA vaccine, elicited robust Spike-specific antibody and T-cell responses which has also translated into efficacy in protecting transgenic mice from SARS-CoV-2. Calculations were performed using the SAS V9.4 software (SAS Institute Inc., Cary, NC, USA). Viral RNA was extracted from 140l serum and tissue samples using the QIAamp viral RNA mini kit (QIAGEN, Hilden, Germany). 5a). SD; standard deviation. This was concordant with the previous findings that Omicron subvariants could evade NAb induced by the first-generation or WT-virus-based vaccines46. Note; the IgG2a/IgG1 ratio of 10g and 30g immunized mice were not analyzed due to limited volume of serum samples. Jackson, L. A. et al. Internet Explorer). Nature 584, 450456 (2020). A table of quantitative anti-spike levels for otherwise healthy, recently vaccinated individuals by week of vaccination to aid in interpretation of test results is available in Table 3 in this pre-print. The use of antibody therapy for PrEP, which is the use of medications to prevent infection before exposure to a virus, is currently being studied for its potential efficacy in immunocompromised individuals with COVID-19. New crop of COVID-19 mRNA vaccines could be easier to store, cheaper to use: Science [updated 5 April 2022; cited 30 August 2022]. However, at week 2 after the first dose, 6/6 and 4/6 animals from the 10g and 1g groups, respectively, showed a dose-dependent manner of NAb response to vaccine administration. Hunsawong, T. et al. This was followed by exposure to the same S1 concentration for seven consecutive days.
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